Different Diagnostic Tests Using Serum Antibodies

Describe in detail how a technician performs each of the quest diagnostic tests with a sample of blood serum that contains specific antibodies, and bespeak the advantages and disadvantages associated with each test . (1) the balance obsession test, (2) the light antibody test, (3) the radioimmunoassay, and the enzyme-linked immunosorbent assay. The co-occurrence fixation test is used to detect antibodies for some(prenominal) types of viruses, fungi, and bacterium involves a two step process.. The first come out or test system utilizes the longanimouss serum, a grooming of antigen from the suspected pathogen, and equilibrise from guinea go throughs. The uphold part, the indicator system, uses sheep blushful strain cells and prepa ruby antibodies that recognize the sheep crimson blood cells. The soft touch step involves heating the patients serum to undo any existing complement in the serum. The antigen and guinea pig complement are accordingly added t o the serum, and past incubated at 37 degrees Celsius for 90 minutes. An antibody-antigen interaction occurs if thither are antibodies specific for the antigen present. The complement is wintry, or used up, still there is no sign signifying that a reaction has interpreted smirch. afterward the indicator is added the supply is reincubated.

If the complement was already fixed then the lysis of sheep red blood cells would not take place and the cells would remain built-in. After the sample is centrifuged, a clear smooth with blood cells at the arsehole is observed. It can be conclude that the patients serum contained antibodies. If the complement was! not fixed, then the sheep cells will be forthcoming to react and they will lyse. After the sample is centrifuged, a red fluid with no intact blood cells at the render is observed and it can be concluded that the serum lacked antibodies. 1 advantage of the complement fixation test is that it can be used to detect a variety of antibodies by change the antigen. Tests can be conducted for diseases such(prenominal) as encephalitis,...If you want to demoralise a full essay, differentiate it on our website: BestEssayCheap.com

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